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National Institutes of Health Office of Dietary Supplements
Nutrient Biomarkers Analytical Methodology: Vitamin D Workshop

Bethesda North Marriott Hotel & Conference Center, Bethesda, MD

December 16, 2009

7:30 - 8:00Registration and Continental Breakfast
8:00 - 8:15Welcome
Paul M. Coates, Director, Office of Dietary Supplements
Round Table Introductions
John H. Cardellina II, Office of Dietary Supplements
Charge to speakers and observers
Joseph M. Betz, Office of Dietary Supplements
8:15 - 8:35Why vitamin D?
Mary Frances Picciano, Office of Dietary Supplements

Opening Session:
8:35 - 8:55Vitamin D Chemistry and metabolites
Hector DeLuca, University of Wisconsin
8:55 – 9:15The significance of 3-epi-25-hydroxyvitamin D2 and 3-epi-25-hydroxyvitamin D3 in clinical measurements of vitamin D exposure
G. Satya Reddy, Brown University
9:15 – 9:35In an ideal world, we'd be counting molecules
Stephen Barnes, University of Alabama at Birmingham
9:35 - 10:15Discussion
10:15 - 10:45Mid-morning Break

Session 1: General Principles of Measurement Science
10:45 - 11:25Fitness for purpose, accuracy, and precision. Establishing the "trueness" of analytical measurements, evaluating reliability of the method, and determining inherent variability of the method
Mark Roman, Tampa Bay Analytical Research, Inc.
11:25 - 11:45Reference materials for clinical measurands: the importance of commutability
John H. Eckfeldt, University of Minnesota
11:45 - 12:15Discussion
12:15 - 1:30Lunch (on your own)

Session 2: Analytical Approaches to Nutrient Measurement
1:30 - 1:50Physical and bioorganic principles of antigen/antibody measurements and measurement implications - Accuracy, Precision, Specificity
Gyorgy Abel, Director, Lahey Clinic
1:50 - 2:10Physical and bioorganic principles of chromatographic measurements using spectrophotometric/spectrometric detection - Accuracy, Precision, Specificity
Craig Byrdwell, USDA, ARS, BHNRC, FCMDL
2:10 - 2:30Assessment of available analytical methods for vitamin D measurement in biological fluids
Graham D. Carter, DEQAS Organizer, Imperial College Healthcare, London
2:30 - 2:50Standard Reference Materials for vitamin D measurement in biological fluids
Karen Phinney, NIST
2:50 – 3:20Discussion
3:20 - 3:40Mid-Afternoon Break

Closing Session:
3:40 - 4:00Approaches to reconciling measurement methods based on different physical/chemical principles
Linda Thienpont, University of Ghent, Belgium
4:00 - 4:20Discussion
4:20 - 5:00Summary and identification of action items: knowledge gaps, and suggested research agenda for future studies
5:00 – 5:30Public Comment
5:30 PMAdjourn