National Institutes of Health Office of Dietary Supplements
Nutrient Biomarkers Analytical Methodology: Vitamin D Workshop

Bethesda North Marriott Hotel & Conference Center, Bethesda, MD

December 16, 2009

7:30 - 8:00	Registration and Continental Breakfast
8:00 - 8:15	Welcome Paul M. Coates, Director, Office of Dietary Supplements
	Round Table Introductions John H. Cardellina II, Office of Dietary Supplements
	Charge to speakers and observers Joseph M. Betz, Office of Dietary Supplements
8:15 - 8:35	Why vitamin D? Mary Frances Picciano, Office of Dietary Supplements
Opening Session:
8:35 - 8:55	Vitamin D Chemistry and metabolites Hector DeLuca, University of Wisconsin
8:55 – 9:15	The significance of 3-epi-25-hydroxyvitamin D2 and 3-epi-25-hydroxyvitamin D3 in clinical measurements of vitamin D exposure G. Satya Reddy, Brown University
9:15 – 9:35	In an ideal world, we'd be counting molecules Stephen Barnes, University of Alabama at Birmingham
9:35 - 10:15	Discussion
10:15 - 10:45	Mid-morning Break
Session 1: General Principles of Measurement Science
10:45 - 11:25	Fitness for purpose, accuracy, and precision. Establishing the "trueness" of analytical measurements, evaluating reliability of the method, and determining inherent variability of the method Mark Roman, Tampa Bay Analytical Research, Inc.
11:25 - 11:45	Reference materials for clinical measurands: the importance of commutability John H. Eckfeldt, University of Minnesota
11:45 - 12:15	Discussion
12:15 - 1:30	Lunch (on your own)
Session 2: Analytical Approaches to Nutrient Measurement
1:30 - 1:50	Physical and bioorganic principles of antigen/antibody measurements and measurement implications - Accuracy, Precision, Specificity Gyorgy Abel, Director, Lahey Clinic
1:50 - 2:10	Physical and bioorganic principles of chromatographic measurements using spectrophotometric/spectrometric detection - Accuracy, Precision, Specificity Craig Byrdwell, USDA, ARS, BHNRC, FCMDL
2:10 - 2:30	Assessment of available analytical methods for vitamin D measurement in biological fluids Graham D. Carter, DEQAS Organizer, Imperial College Healthcare, London
2:30 - 2:50	Standard Reference Materials for vitamin D measurement in biological fluids Karen Phinney, NIST
2:50 – 3:20	Discussion
3:20 - 3:40	Mid-Afternoon Break
Closing Session:
3:40 - 4:00	Approaches to reconciling measurement methods based on different physical/chemical principles Linda Thienpont, University of Ghent, Belgium
4:00 - 4:20	Discussion
4:20 - 5:00	Summary and identification of action items: knowledge gaps, and suggested research agenda for future studies
5:00 – 5:30	Public Comment
5:30 PM	Adjourn