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Dietary Supplement Analytical Methods and Reference Materials Program (AMRM)

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Analytical Methods for Dietary Supplements

scientist examining herb leavesAnalytical methods program area focuses on the development and validation of analytical methods that can be used to verify dietary ingredient and label content claims, as tools to meet dietary supplement current good manufacturing practice requirements, and to determine the amounts of constituents of dietary supplements. Current method development efforts favor methods for analytes with high potential public health significance.

Method development

  • The AMRM Program aims to promote analytical method development in prioritized areas to address current knowledge gaps.  Funded projects have focused on the development of non-targeted methods for botanical classification and authentication, and targeted methods to identify and quantify adulterants, natural toxins, pesticides and toxic elements.

Method validation 

  • From 2013 to 2018 community-driven method validation activities were coordinated by AOAC International through a stakeholder panel funded through a contract from ODS. Information about the AOAC Stakeholder Panel on Dietary Supplements, including the resulting analytical method publications, is available on the AOAC website.external link disclaimer
  • AOAC International has published guidelines for performing a single laboratory validation study for quantitive chemical methods for dietary supplements and guidelines for the validation of methods to identify botanical dietary supplements in the 2013 edition of the AOAC Official Methods of Analysis, Appendix K:  Guidelines for Dietary Supplements and Botanicalsexternal link disclaimer
  • NIH Administrative Supplement funding awards for single laboratory validation of quantitative analytical methods:  In an attempt to leverage basic biomedical research already supported by NIH and to capture and validate new methods when first developed, AMRM makes available competitive administrative supplement funding to certain active NIH awards via PA 18-818external link disclaimer.


AMRM Supported AOAC International methods of analysis for dietary ingredients and supplements
AOAC guidelines for dietary supplement and botanical method validationexternal link disclaimer

Method Type

Analyte(s) in raw materials, dietary ingredients, and supplements

OMA[1]

SMPR[2]

Quant

Glucosamine

2005.01external link disclaimer

N/A

Quant

b-carotene

2005.07external link disclaimer

N/A

Quant

flavonol aglycones from Ginkgo biloba

2006.07external link disclaimer

N/A

Quant

Campesterol, stigmasterol, and b-sitosterol from Serenoa repens (saw palmetto)

2007.03external link disclaimer

N/A

Quant

Aristolochic acid I

2007.05external link disclaimer

N/A

Quant

Aflatoxins B1, B2, G1 and G2 and ochratoxin A in ginseng and ginger

2008.02external link disclaimer

N/A

Quant

Total soy isoflavones

2008.03external link disclaimer

N/A

Quant

Hydrastine and berberine from Hydrastis canadensis (goldenseal)

2008.04external link disclaimer

N/A

Quant

Coenzyme Q10

2008.07external link disclaimer

N/A

Quant

Aconitum alkaloids

2008.11external link disclaimer

N/A

Quant

Lycopene

2009.04external link disclaimer

N/A

Quant

Total chondroitin sulfate

2015.11external link disclaimer

2014.009external link disclaimer

ID

Phosphodiesterase Type 5 (PDE5) inhibitors

2015.12external link disclaimer

2014.010external link disclaimer

Quant

Withanolides from Withania somnifera (ashwagandha)

2015.17external link disclaimer

2015.007external link disclaimer

Quant

Aloin A, aloin B, and aloe-emodin

2016.09external link disclaimer

2015.015external link disclaimer

Quant

Theanine from Camellia sinensis (tea)

2016.10external link disclaimer

2015.014external link disclaimer

Quant

Curcumin, demethoxycurcumin, bisdemethyloxycurcumin from Curcuma longa

2016.16external link disclaimer

2016.003external link disclaimer

ID

Milk proteins

2017.12external link disclaimer

2016.015external link disclaimer

Quant

Total phenolic content in extracts

2017.13external link disclaimer

2015.009external link disclaimer

ID

Pea, rice, soy proteins

2017.11external link disclaimer

2016.016external link disclaimer

Quant

Mitragynine, 7-hydroxymitragynine, alkaloids from Mitragyna speciosa (kratom)

2017.14external link disclaimer

2015.008external link disclaimer

Quant

Gingerols and shogaols in ginger

2018.04external link disclaimer

2017.012external link disclaimer

Quant

Phenolic compounds in Echinacea

2018.08external link disclaimer

2017.015external link disclaimer

Quant

Select ginsenosides in ginseng

2018.09external link disclaimer

2017.014external link disclaimer

Quant

Aloe vera polysaccharides

2018.14external link disclaimer

2017.009external link disclaimer

Quant

Select kavalactones and flavokavains in kava

-

2018.005external link disclaimer

Quant

Vitamin B12

-

2016.017 external link disclaimer

 

[1] AOAC International Official Method of Analysis: https://www.aoac.org/scientific-solutions/standards-and-official-methods/external link disclaimer

[2] AOAC International Standard Method Performance Requirements: http://www.eoma.aoac.org/app_f.pdfexternal link disclaimer