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Dietary Supplement Analytical Methods and Reference Materials Program (AMRM)

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Reference Materials for Dietary Supplement Analysis

NIST Standard Reference Material vials for omega-3 fatty acidsReference materials (RMs) are important resources for optimizing and verifying analytical measurements. An RM is a material that is homogenous and stable with respect to one or more specified properties. For food and dietary supplement RMs, the specified properties are often quantified values with uncertainties for select macro- and micronutrients, marker compounds, toxins, contaminants, and/or adulterants (1). Matrix-based and calibration RMs are therefore powerful tools for developing new analytical methods, verifying measurements of unknown samples, supporting quality assurance, and performing instrument calibrations.   

The AMRM Program makes available certified reference materials (CRMs), primarily through collaborative work with the National Institute of Standards and Technology (NIST)external link disclaimer, in which the target constituents are generally quantified by multiple analytical methods. In developing CRMs for botanical dietary supplements, NIST often engages with private sector suppliers and manufacturers to procure kilogram quantities of authenticated natural-matrix materials (e.g., vouchered whole plants or powdered plant parts). To the extent possible, NIST dietary supplement CRMs are designed as suites of materials representing the different manufacturing stages, such as a plant part (e.g., leaves or roots), an extract, and an oral dosage formulation.  To date, the AMRM Program has worked with NIST to develop more than 30 dietary supplement CRMsexternal link disclaimer.

NIH and NIST identify and prioritize needs for RMs by assessing biomedical research, methodological gaps, safety concerns, and market reports. AMRM-produced CRMs for dietary ingredients and supplements are not intended to represent an ideal or “gold standard” preparation. Although the CRMs must be derived from authenticated sources, their composition is not intended to set an industry standard, meet a given company’s specifications, or elicit a specific biological response. Rather, AMRM-produced CRMs are designed to represent the types of challenges that analysts might encounter with real-world samples when verifying ingredients or formulations or measuring clinical samples.

Through expanded use of RMs, dietary supplement stakeholders in academia, government, and industry can better advance biomedical research, protect public safety, ensure quality control, and promote public health (2).

1. SA Wise and MM Phillips. 2019. Anal Bioanal Chem 411(1):97-127external link disclaimer.
2. CA Rimmer, KE Sharpless, SA Wise, JM Betz, PM Coates. 2013. Anal Bioanal Chem 405(13):4337-44external link disclaimer.

The NIST Web site provides additional information about the dietary supplement reference materials program:

AMRM-supported CRMs for dietary supplement analyses have quantified values with uncertainties for select macro- and micronutrients, marker compounds, toxins, contaminants, and/or adulterants. These calibration solutions and matrix-based CRMs are powerful tools for developing new analytical methods, verifying measurements of unknown samples, supporting quality assurance, and performing instrument calibrations.

Provider

Catalog Link

Description

Analytes with Certified or Reference Values

Note

NIST

SRM 3291external link disclaimer

Bilberry Extract

organic acids, anions

NIST

SRM 3274external link disclaimer

Botanical Oils Containing Omega-3 and Omega-6 Fatty Acids

select fatty acids

NIST

SRM 3532external link disclaimer

Calcium-Containing Solid Oral Dosage Form

vitamins, elements

NIST

SRM 3279external link disclaimer

Chromium Dietary Supplement

chromium, vanadium,

NEW

NIST

SRM 3281external link disclaimer

Cranberry (Fruit)

organic acids, sugars, elements, carbohydrates, antioxidant capacity

NIST

SRM 3283external link disclaimer

Cranberry Extract

organic acids, antioxidant capacity

NIST

SRM 3284external link disclaimer

Cranberry-Containing Solid Oral Dosage Form

organic acids, antioxidant capacity

NIST

SRM 3247external link disclaimer

Ginkgo biloba (Extract)

flavonoid aglycones, terpene lactones (Ginkgolides), elements

NIST

SRM 3246external link disclaimer

Ginkgo biloba (Leaves)

flavonoid aglycones, terpene lactones (Ginkgolides), elements, DNA sequence

NIST

SRM 3248external link disclaimer

Ginkgo-Containing Tablets

flavonoid aglycones, terpene lactones (Ginkgolides), elements

Cerilliant

G-027external link disclaimer

Ginger Gingerols and Shogaols Mix

6-, 8-, and 10-gingerol and 6-, 8-, and 10-shogaol

NEW

NIST

SRM 3255external link disclaimer

Green Tea (Camellia sinensis) Extract

caffeine, catechins, theobromine, elements, amino acids

NIST

SRM 3254external link disclaimer

Green Tea (Camellia sinensis) Leaves

caffeine, catechins, theobromine, elements, amino acids

NIST

SRM 3256external link disclaimer

Green Tea-Containing Solid Oral Dosage Form

caffeine, catechins, theobromine, elements, amino acids

NIST

SRM 3299external link disclaimer

Ground Turmeric (Curcuma longa L.) Rhizome

curcuminoids

NEW

NIST

SRM 3530external link disclaimer

Iodized Table Salt (Iodide)

elements

Cerilliant

K-007external link disclaimer

Kava Kavalactones Mix

select kavalactones and flavokavains

NEW

NIST

SRM 3232external link disclaimer

Kelp Powder (Thallus laminariae)

vitamins, elements

NIST

RM-8037external link disclaimer

Krill Oil

fatty acids

NEW

NIST

SRM 3282external link disclaimer

Low-Calorie Cranberry Juice Cocktail

organic acids, sugars, elements, phosphate, sulfate

NIST

SRM 3285external link disclaimer

Mixed-Berry Containing Solid Oral Dosage Form

organic acids, antioxidant capacity

NIST

SRM 3280external link disclaimer

Multivitamin/Multielement Tablets

vitamins, elements, carotenoids

NIST

SRM 3275external link disclaimer

Omega-3 and Omega-6 Fatty Acids in Fish Oil

fatty acids

NIST

SRM 3251external link disclaimer

Saw Palmetto (Serenoa repens) Extract

fatty acids, carotenoids

NIST

SRM 3250external link disclaimer

Saw Palmetto (Serenoa repens) Fruit

fatty acids

NIST

SRM 3235external link disclaimer

Soy Milk

vitamins, fatty acids, elements, amino acids

NIST

SRM 3237external link disclaimer

Soy Protein Concentrate

daidzin, genistin, glycitin

NIST

SRM 3236external link disclaimer

Soy Protein Isolate

flavonoid aglycones, isoflavones

NIST

SRM 3238external link disclaimer

Soy-Containing Solid Oral Dosage Form

flavonoid aglycones, isoflavones

NIST

SRM 3262external link disclaimer

St. John's Wort (Hypericum perforatum L.) Aerial Parts

flavonoid aglycones, elements, DNA sequence

NIST

SRM 3253external link disclaimer

Yerba Mate Leaves

polycyclic aromatic hydrocarbons (PAHs), proximates

NIST

SRM 3266external link disclaimer

Hypericin Calibration Solution

hypericin

Currently unavailable

NIST

SRM 3239external link disclaimer

Isoflavones Calibration Solutions

daidzin, daidzein, genistin, genistein, glycitin, glycitein

Currently unavailable

NIST

SRM 3286external link disclaimer

Organic Acids Calibration Solution

citric, malic, quinic, shikimic, tartaric, and fumaric acids

Currently unavailable

NIST

SRM 3258external link disclaimer

Bitter Orange (Fruit)

synephrine, select citrus alkaloids, octopamine, arsenic, cadmium, lead

Discontinued

NIST

SRM 3261external link disclaimer

Bitter Orange Dietary Supplement Suite

synephrine, select citrus alkaloids, octopamine, hordenine, caffeine, arsenic, cadmium, lead

Discontinued

NIST

SRM 3259external link disclaimer

Bitter Orange Extract

synephrine, select citrus alkaloids, octopamine, hordenine, arsenic, cadmium, lead

Discontinued

NIST

SRM 3260external link disclaimer

Bitter Orange-Containing Solid Oral Dosage Form

synephrine, select citrus alkaloids, octopamine, hordenine, caffeine, arsenic, cadmium, lead

Discontinued

NIST

SRM 3257external link disclaimer

Catechin Calibration Solutions

catechin, gallocatechins, epicatechins, EGCG

Discontinued

NIST

SRM 3245external link disclaimer

Ephedra Dietary Supplement Suite

ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, caffeine, vitamins, elements, amino acids, fatty acids, proximites

Discontinued

NIST

SRM 3240external link disclaimer

Ephedra sinica Stapf Aerial Parts

ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, elements

Discontinued

NIST

SRM 3242external link disclaimer

Ephedra sinica Stapf Commercial Extract

ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, elements

Discontinued

NIST

SRM 3241external link disclaimer

Ephedra sinica Stapf Native Extract

ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, elements

Discontinued

NIST

SRM 3244external link disclaimer

Ephedra-Containing Protein Powder

ephedrine, select ephedrine alkaloids, total alkaloids, caffeine, vitamins, elements, amino acids, fatty acids, proximites

Discontinued

NIST

SRM 3243external link disclaimer

Ephedra-Containing Solid Oral Dosage Form

ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, elements

Discontinued

NIST

SRM 3249external link disclaimer

Ginkgo Dietary Supplement Suite

quercitin, kaempferol, isorhamnetin, total aglycones, ginkgolides, bilobalide, total terpene lactones, arsenic, cadmium, lead, mercury

Discontinued