Dietary Supplement Analytical Methods and Reference Materials Program (AMRM)

AMRM Home | AMRM Overview | AMRM Program Areas | Organizations & Resources | FAQ | Glossary

Reference Materials for Dietary Supplement Analysis

NIST Standard Reference Material vials for omega-3 fatty acidsReference materials (RMs) are important resources for optimizing and verifying analytical measurements. An RM is a material that is homogenous and stable with respect to one or more specified properties. For food and dietary supplement RMs, the specified properties are often quantified values with uncertainties for select macro- and micronutrients, marker compounds, toxins, contaminants, and/or adulterants (1). Matrix-based and calibration RMs are therefore powerful tools for developing new analytical methods, verifying measurements of unknown samples, supporting quality assurance, and performing instrument calibrations.   

The AMRM Program makes available certified reference materials (CRMs), primarily through collaborative work with the National Institute of Standards and Technology (NIST)external link disclaimer, in which the target constituents are generally quantified by multiple analytical methods. In developing CRMs for botanical dietary supplements, NIST often engages with private sector suppliers and manufacturers to procure kilogram quantities of authenticated natural-matrix materials (e.g., vouchered whole plants or powdered plant parts). To the extent possible, NIST dietary supplement CRMs are designed as suites of materials representing the different manufacturing stages, such as a plant part (e.g., leaves or roots), an extract, and an oral dosage formulation.  To date, the AMRM Program has worked with NIST to develop more than 30 dietary supplement CRMsexternal link disclaimer.

NIH and NIST identify and prioritize needs for RMs by assessing biomedical research, methodological gaps, safety concerns, and market reports. AMRM-produced CRMs for dietary ingredients and supplements are not intended to represent an ideal or “gold standard” preparation. Although the CRMs must be derived from authenticated sources, their composition is not intended to set an industry standard, meet a given company’s specifications, or elicit a specific biological response. Rather, AMRM-produced CRMs are designed to represent the types of challenges that analysts might encounter with real-world samples when verifying ingredients or formulations or measuring clinical samples.

Through expanded use of RMs, dietary supplement stakeholders in academia, government, and industry can better advance biomedical research, protect public safety, ensure quality control, and promote public health (2).

1. SA Wise and MM Phillips. 2019. Anal Bioanal Chem 411(1):97-127.
2. CA Rimmer, KE Sharpless, SA Wise, JM Betz, PM Coates. 2013. Anal Bioanal Chem 405(13):4337-44.

The NIST Web site provides additional information about the dietary supplement reference materials program:

AMRM-supported CRMs for dietary supplement analyses have quantified values with uncertainties for select macro- and micronutrients, marker compounds, toxins, contaminants, and/or adulterants. These calibration solutions and matrix-based CRMs are powerful tools for developing new analytical methods, verifying measurements of unknown samples, supporting quality assurance, and performing instrument calibrations.

Provider Catalog Link Description Analytes with Certified or Reference Values Note
Cerilliant A-197external link disclaimer Ashwagandha Dietary Ingredients Mix withaferin A, withanolide A, withanolide B, withanoside IV, withanoside V NEW
Cerilliant A-204external link disclaimer 12-deoxywithastramonolide 12-Deoxywithastramonolide NEW
Cerilliant A-205external link disclaimer Withanone withanone NEW
NIST SRM 3291external link disclaimer Bilberry Extract organic acids, anions
NIST SRM 3274external link disclaimer Botanical Oils Containing Omega-3 and Omega-6 Fatty Acids select fatty acids
NIST SRM 3279external link disclaimer Chromium Dietary Supplement chromium, vanadium
NIST SRM 3281external link disclaimer Cranberry (Fruit) organic acids, sugars, elements, carbohydrates, antioxidant capacity
NIST SRM 3283external link disclaimer Cranberry Extract organic acids, antioxidant capacity
NIST SRM 3284external link disclaimer Cranberry-Containing Solid Oral Dosage Form organic acids, antioxidant capacity
NIST SRM 3247external link disclaimer Ginkgo biloba (Extract) flavonoid aglycones, terpene lactones (Ginkgolides), elements
NIST SRM 3246external link disclaimer Ginkgo biloba (Leaves) flavonoid aglycones, terpene lactones (Ginkgolides), elements, DNA sequence
NIST SRM 3248external link disclaimer Ginkgo-Containing Tablets flavonoid aglycones, terpene lactones (Ginkgolides), elements
Cerilliant G-027external link disclaimer Ginger Gingerols and Shogaols Mix 6-, 8-, and 10-gingerol; 6-, 8-, and 10-shogaol
NIST SRM 3398external link disclaimer Ginger (Zingiber officinale) Rhizome 6-, 8-, and 10-gingerol; 6-, 8-, and 10-shogaol; As; Pb; Hg
NIST SRM 8666external link disclaimer Ginger (Zingiber officinale) Extract 6-, 8-, and 10-gingerol; 6-, 8-, and 10-shogaol; As; Pb; Hg
NIST SRM 3384external link disclaimer Ground Asian Ginseng (Panax ginseng) Rhizome ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1, toxic elements, DNA NEW
NIST SRM 3385external link disclaimer Asian Ginseng (Panax ginseng) Extract ginsenosides Rb1, Rb2, Rd, Re, Rf, Rg1, toxic elements NEW
NIST RM 8664external link disclaimer Ginseng-Containing Solid Oral Dosage Form ginsenosides Rb1, Rb2, Rc, Rd, Re, Rf, Rg1 NEW
NIST SRM 3389external link disclaimer Ginsenosides Calibration Solutions ginsenosides Rg1, Re, Rb1, Rc, Rb2, Rd
NIST SRM 3255external link disclaimer Green Tea (Camellia sinensis) Extract caffeine, catechins, theobromine, elements, amino acids
NIST SRM 3254external link disclaimer Green Tea (Camellia sinensis) Leaves caffeine, catechins, theobromine, elements, amino acids
NIST SRM 3256external link disclaimer Green Tea-Containing Solid Oral Dosage Form caffeine, catechins, theobromine, elements, amino acids
NIST RM 8650external link disclaimer Ground Kudzu (Pueraria montana var. lobata) Rhizome daidzein, daidzin, puerarin, toxic elements, DNA NEW
NIST SRM 3268external link disclaimer Kudzu (Pueraria montana var. lobata) Extract daidzein, daidzin, puerarin, toxic elements NEW
NIST RM 8652external link disclaimer Kudzu-Containing Solid Oral Dosage Form daidzein, daidzin, puerarin NEW
NIST SRM 3299external link disclaimer Ground Turmeric (Curcuma longa L.) Rhizome curcuminoids
NIST SRM 3530external link disclaimer Iodized Table Salt (Iodide) elements
Cerilliant K-007external link disclaimer Kava Kavalactones Mix select kavalactones and flavokavains
NIST SRM 3232external link disclaimer Kelp Powder (Thallus laminariae) vitamins, elements
NIST RM 8037external link disclaimer Krill Oil fatty acids
NIST SRM 3282external link disclaimer Low-Calorie Cranberry Juice Cocktail organic acids, sugars, elements, phosphate, sulfate
NIST SRM 3285external link disclaimer Mixed-Berry Containing Solid Oral Dosage Form organic acids, antioxidant capacity
NIST SRM 3289external link disclaimer Multivitamin Tablets water-soluble vitamins NEW
NIST SRM 3294external link disclaimer Multielement Tablets nutritional elements NEW
NIST SRM 3275external link disclaimer Omega-3 and Omega-6 Fatty Acids in Fish Oil fatty acids
NIST SRM 3251external link disclaimer Saw Palmetto (Serenoa repens) Extract fatty acids, carotenoids
NIST SRM 3250external link disclaimer Saw Palmetto (Serenoa repens) Fruit fatty acids
NIST SRM 3235external link disclaimer Soy Milk vitamins, fatty acids, elements, amino acids
NIST SRM 3237external link disclaimer Soy Protein Concentrate daidzin, genistin, glycitin
NIST SRM 3236external link disclaimer Soy Protein Isolate flavonoid aglycones, isoflavones
NIST SRM 3238external link disclaimer Soy-Containing Solid Oral Dosage Form flavonoid aglycones, isoflavones
NIST SRM 3262external link disclaimer St. John's Wort (Hypericum perforatum L.) Aerial Parts flavonoid aglycones, elements, DNA sequence
NIST SRM 3253external link disclaimer Yerba Mate Leaves polycyclic aromatic hydrocarbons (PAHs), proximates
NIST SRM 3266external link disclaimer Hypericin Calibration Solution hypericin Currently unavailable
NIST SRM 3239external link disclaimer Isoflavones Calibration Solutions daidzin, daidzein, genistin, genistein, glycitin, glycitein Currently unavailable
NIST SRM 3286external link disclaimer Organic Acids Calibration Solution citric, malic, quinic, shikimic, tartaric, and fumaric acids Currently unavailable
NIST SRM 3280external link disclaimer Multivitamin/Multielement Tablets vitamins, elements, carotenoids Discontinued
NIST SRM 3532external link disclaimer Calcium-Containing Solid Oral Dosage Form vitamins, elements Discontinued
NIST SRM 3258external link disclaimer Bitter Orange (Fruit) synephrine, select citrus alkaloids, octopamine, arsenic, cadmium, lead Discontinued
NIST SRM 3261external link disclaimer Bitter Orange Dietary Supplement Suite synephrine, select citrus alkaloids, octopamine, hordenine, caffeine, arsenic, cadmium, lead Discontinued
NIST SRM 3259external link disclaimer Bitter Orange Extract synephrine, select citrus alkaloids, octopamine, hordenine, arsenic, cadmium, lead Discontinued
NIST SRM 3260external link disclaimer Bitter Orange-Containing Solid Oral Dosage Form synephrine, select citrus alkaloids, octopamine, hordenine, caffeine, arsenic, cadmium, lead Discontinued
NIST SRM 3257external link disclaimer Catechin Calibration Solutions catechin, gallocatechins, epicatechins, EGCG Discontinued
NIST SRM 3245external link disclaimer Ephedra Dietary Supplement Suite ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, caffeine, vitamins, elements, amino acids, fatty acids, proximites Discontinued
NIST SRM 3240external link disclaimer Ephedra sinica Stapf Aerial Parts ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, elements Discontinued
NIST SRM 3242external link disclaimer Ephedra sinica Stapf Commercial Extract ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, elements Discontinued
NIST SRM 3241external link disclaimer Ephedra sinica Stapf Native Extract ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, elements Discontinued
NIST SRM 3244external link disclaimer Ephedra-Containing Protein Powder ephedrine, select ephedrine alkaloids, total alkaloids, caffeine, vitamins, elements, amino acids, fatty acids, proximites Discontinued
NIST SRM 3243external link disclaimer Ephedra-Containing Solid Oral Dosage Form ephedrine, select ephedrine alkaloids, total alkaloids, arsenic, cadmium, mercury, elements Discontinued
NIST SRM 3249external link disclaimer Ginkgo Dietary Supplement Suite quercitin, kaempferol, isorhamnetin, total aglycones, ginkgolides, bilobalide, total terpene lactones, arsenic, cadmium, lead, mercury Discontinued